Canonical pathway and network analysis

Canonical pathways. At 3 mo, 1 1 metabolic and 7 signaling pathways Bead bracelet significantly perturbed by the WD and at 6 mo, 8 signaling (Fig. IA) and 3 metabolic pathways were significantly altered (Supplemental Table 6). Pathways altered at both time points included nuclear factor-erythroid 2-related factor (Nrf2)mediated oxidative stress response, aryl hydrocarbon receptor signaling, insulin-like growth factor 1 signaling, Iipopolysaccharide /interleukin !-mediated inhibition of retinoid X receptor function, circadian rhythm signaling, and GSH metabolism. Xenobiotic metabolism signaling at 3 mo, T- and B-cell receptor signaling, and the complement coagulation cascade at 6 mo were altered. Metabolism of xenobiotics by cytochrome P450, butanoate metabolism, and fatty acid metabolism were Tiffany Notes ring at 3 mo (Supplemental Table 6). Arachidonic acid metabolism was among the significantly upregulated pathways at 6 mo (Fig. 1β). Downregulated pathways at 3 mo included Gl/S checkpoint regulation (genes CCND2, E2F2) and nuclear-factor-κB signaling and at 6 mo, T- and B-cell receptor signaling and complement and coagulation cascades (Supplemental Table 6).

Biologically relevant networks. Differentially Elsa Peretti Open Heart ring genes grouped into networks associated with lipid metabolism, cellular compromise such as free radical scavenging, circadian rhythm, cellular growth, and proliferation (Supplemental Table 7). Network representations led to identification of a c-Fos and J un B proto-oncogene-centered network induced by the WD (Fig. 2).